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Lignin-modifying enzymes from selected white-rot fungi: production and role from in lignin degradation

Annele Hatakka
DOI: http://dx.doi.org/10.1111/j.1574-6976.1994.tb00039.x 125-135 First published online: 1 March 1994


White-rot fungi produce extracellular lignin-modifying enzymes, the best characterized of which are laccase (EC, lignin peroxidases (EC and manganese peroxidases (EC Lignin biodegradation studies have been carried out mostly using the white-rot fungus Phanerochaete chrysosporium which produces multiple isoenzymes of lignin peroxidase and manganese peroxidase but does not produce laccase. Many other white-rot fungi produce laccase in addition to lignin and manganese peroxidases and in varying combinations. Based on the enzyme production patterns of an array of white-rot fungi, three categories of fungi are suggested: (i) lignin-manganese peroxidase group (e.g.P. chrysosporium and Phlebia radiata), (ii) manganese peroxidase-laccase group (e.g. Dichomitus squalens and Rigidoporus lignosus), and (iii) lignin peroxidase-laccase group (e.g. Phlebia ochraceofulva and Junghuhnia separabilima). The most efficient lignin degraders, estimated by 14CO2 evolution from 14C-[Ring]-labelled synthetic lignin (DHP), belong to the first group, whereas many of the most selective lignin-degrading fungi belong to the second, although only moderate to good [14C]DHP mineralization is obtained using fungi from this group. The lignin peroxidase-laccase fungi only poorly degrade [14C]DHP.

Key words
  • Lignin
  • White-rot fungi
  • Lignin peroxidase
  • Manganese peroxidase
  • Laccase
  • Enzyme regulation

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